As much as seventy-five p.c of sufferers handled for most cancers undergo from cognitive deficits which may persist for months to many years, severely impairing high quality of life. Though the variety of most cancers survivors is growing tremendously, no efficacious interventions exist. Cisplatin, mostly employed for strong tumors, results in cognitive impairment together with deficits in reminiscence and govt functioning. We not too long ago proposed poor neuronal mitochondrial perform as its underlying mechanism. We hypothesized nasal administration of mitochondria remoted from human mesenchymal stem cells to mice, can reverse cisplatin-induced cognitive deficits.
Puzzle field, novel object place recognition and Y-maze checks had been used to evaluate the cognitive perform of mice. Immunofluorescence and high-resolution confocal microscopy had been employed to hint the nasally delivered mitochondria and consider their impact on synaptic loss. Black Gold II immunostaining was used to find out myelin integrity. Transmission electron microscopy helped decide mitochondrial and membrane integrity of mind synaptosomes. RNA-sequencing was carried out to analyse the hippocampal transcriptome.
Two nasal administrations of mitochondria remoted from human mesenchymal stem cells to mice, restored govt functioning, working and spatial reminiscence. Confocal imaging revealed nasally delivered mitochondria quickly arrived within the meninges the place they had been readily internalized by macrophages. The administered mitochondria additionally accessed the rostral migratory stream and varied different mind areas together with the hippocampus the place they colocalized with GFAP+ cells. The restoration of cognitive perform was related to structural restore of myelin within the cingulate cortex and synaptic loss within the hippocampus. Nasal mitochondrial donation additionally reversed the underlying synaptosomal mitochondrial defects. Furthermore, transcriptome evaluation by RNA-sequencing confirmed reversal of cisplatin-induced adjustments within the expression of about seven hundred genes within the hippocampus. Pathway evaluation recognized Nrf2-mediated response as the highest canonical pathway
Our outcomes present key proof on the therapeutic potential of remoted mitochondria – restoring each mind construction and performance, their functionality to enter mind meninges and parenchyma upon nasal supply and bear speedy mobile internalization and alter the hippocampal transcriptome. Our information determine nasal administration of mitochondria as an efficient technique for reversing chemotherapy-induced cognitive deficits and restoring mind well being, offering promise for the rising inhabitants of each grownup and pediatric most cancers survivors.
Crystal development inhibition of gypsum beneath regular circumstances and excessive supersaturations by a copolymer of phosphino-polycarboxylic acid
Scale formation is a bottleneck of most industrial and home water gear, specifically, of oilfield water methods. Due to this fact, high-performance and environmentally-benign chemical scale inhibitors are extremely wanted. Phosphino-polycarboxylic acid (PPCA) is a low-in-phosphorous scale inhibitor with excessive inhibition effectivity, however its synthesis and efficiency analyses have been hardly ever disclosed. On this work for the primary time, a PPCA copolymer is synthesized by a easy methodology based mostly on free radical polymerization of acrylic acid and phosphinic acid monomers and straight employed for gypsum scale inhibition. The formation of PPCA was verified by FTIR and 31PNMR spectroscopies, after which its inhibition efficiency was evaluated by the complexometric dedication of the Ca2+ focus.
The PPCA (2.5 ppm) confirmed 100% inhibition effectivity at a saturation index of 0.31 on the room temperature and with out pH regulation after 24 h with virtually no detectable gypsum crystallites even after two months, whereas the business ATMP confirmed a low inhibition effectivity of 30%. The Area Emission Scanning Microscopy (FESEM) pictures of the PPCA-inhibited and uninhibited samples revealed that the everyday gypsum microfibers are distorted and contracted considerably within the inhibited pattern. At a nonetheless increased saturation index of 1.47 (saturation ratio of 10), the inhibition effectivity of PPCA diminished to 16% and 24% for 2 dosages of two.5 and 10 ppm which was attributed to the upper ion exercise coefficients on the extraordinarily excessive ionic power, and therefore, a a lot increased thermodynamic driving power. The speed constants for these two excessive supersaturation circumstances and low PPCA dosages had been additionally calculated and mentioned.
Magnetic nanostructures functionalized with a derived lysine coating utilized to concurrently take away heavy steel pollution from environmental methods
The air pollution of environmental methods with heavy metals is turning into a significant issue worldwide. These contaminants are one of many essential points in sludge (which is taken into account waste) and might even have dangerous results if the sludge shouldn’t be handled correctly. Thus, the event of a novel useful magnetic nanoadsorbent based mostly on a derived lysine is reported right here, which may be effectively utilized for steel removing from sludge. Magnetic nanoparticles had been coated with silica layer and additional modified by covalent bonding of derived lysine.
The morphology of the nanomaterial, its nano-size and the silica layer thickness had been analyzed by transmission electron microscopy. The profitable silanization of the lysine spinoff to the silica-coated magnetic nanostructures was investigated by a number of physicochemical characterization strategies, whereas the magnetic properties had been measured with a vibrating pattern magnetometer. The synthesized nanostructures had been used as adsorbents for simultaneous removing of most crucial heavy metals (Cr, Zn, Cu) from actual advanced sludge suspensions. The principle sensible adsorption parameters, pH of the native stabilized sludge, adsorbent quantity, time, and adsorbent regeneration had been investigated.
 Crystal Violet Solution |
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| CVA500 | ScyTek Laboratories | 500 ml | EUR 106 |
Crystal Violet Solution |
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| CVA999 | ScyTek Laboratories | 1000 ml | EUR 143 |
) Crystal Violet (C.I. 42555) |
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| GA9809-100G | Glentham Life Sciences | 100 g | EUR 62 |
Crystal Violet (C.I. 42555) |
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| GA9809-250G | Glentham Life Sciences | 250 g | EUR 94 |
Crystal Violet (C.I. 42555) |
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| GA9809-25G | Glentham Life Sciences | 25 g | EUR 46 |
Crystal Violet (C.I. 42555) |
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| GA9809-500G | Glentham Life Sciences | 500 g | EUR 142 |
, USP grade) Crystal Violet (C.I. 42555), USP grade |
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| GA0011-100G | Glentham Life Sciences | 100 g | EUR 114 |
Crystal Violet (C.I. 42555), USP grade |
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| GA0011-25G | Glentham Life Sciences | 25 g | EUR 58 |
 Crystal Violet Cell Cytotoxicity Assay Kit |
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| K329-1000 | Biovision | EUR 316 | |
 MACCONKEY AGAR W/O CRYSTAL VIOLET |
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| M13-108-10kg | Alphabiosciences | 10 kg | Ask for price |
MACCONKEY AGAR W/O CRYSTAL VIOLET |
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| M13-108-2kg | Alphabiosciences | 2kg | Ask for price |
MACCONKEY AGAR W/O CRYSTAL VIOLET |
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| M13-108-500g | Alphabiosciences | 500 g | Ask for price |
) Cell Cytotoxicity Assay Kit II (WST-SRB-Crystal Violet) |
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| K173-1000 | Biovision | EUR 707 | |
 20UL VIOLET TIPS FOR V-PREP |
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| VT-20-R | CORNING | 960/pk | EUR 229 |
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Description: Robotic Tips; Robotic tips - Axygen |
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 250UL VIOLET TIPS FOR V-PREP |
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| VT-250-R | CORNING | 960/pk | EUR 229 |
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Description: Robotic Tips; Robotic tips - Axygen |
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250UL VIOLET TIPS FOR V-PREP |
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| VT-250-R-LAB | CORNING | 960/pk | EUR 248 |
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Description: Robotic Tips; Robotic tips - Axygen |
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 50UL VIOLET TIPS FOR V-PREP |
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| VT-50-R | CORNING | 960/pk | EUR 229 |
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Description: Robotic Tips; Robotic tips - Axygen |
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 Fast Red Violet Dye for IHC |
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| G446 | ABM | 250 ml | EUR 342 |
 Leucocrystal violet |
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| HY-D0233 | MedChemExpress | 100mg | EUR 119 |
 Leucocrystal Violet |
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| GT6330-25G | Glentham Life Sciences | 25 g | EUR 102 |
Leucocrystal Violet |
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| GT6330-5G | Glentham Life Sciences | 5 g | EUR 54 |
 Tetrazolium Violet |
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| GT6818-10G | Glentham Life Sciences | 10 g | EUR 293 |
Tetrazolium Violet |
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| GT6818-1G | Glentham Life Sciences | 1 g | EUR 70 |
Tetrazolium Violet |
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| GT6818-5G | Glentham Life Sciences | 5 g | EUR 170 |
 Pyrocatechol Violet |
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| GT7600-25G | Glentham Life Sciences | 25 g | EUR 118 |
Pyrocatechol Violet |
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| GT7600-5G | Glentham Life Sciences | 5 g | EUR 70 |
 Cresyl Violet acetate |
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| HY-101888 | MedChemExpress | 100mg | EUR 119 |
 Cresyl Violet perchlorate |
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| HY-101889 | MedChemExpress | 50mg | EUR 186 |
 0.6ML MICROTUBES. VIOLET |
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| MCT-060-V | CORNING | 1000/pk | EUR 183 |
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Description: Micro Tubes; Micro Tubes Push Cap- Axygen |
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 1.7ML MICROTUBES. VIOLET |
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| MCT-175-V | CORNING | 500/pk | EUR 116 |
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Description: Micro Tubes; Micro Tubes Push Cap- Axygen |
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 Cresyl Violet Acetate |
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| abx082601-10g | Abbexa | 10 g | EUR 620 |
Cresyl Violet Acetate |
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| abx188660-10g | Abbexa | 10 g | EUR 481 |
 Carbol Gentian Violet |
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| CGV500 | ScyTek Laboratories | 500 ml | EUR 106 |
Carbol Gentian Violet |
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| CGV999 | ScyTek Laboratories | 1000 ml | EUR 143 |
Cresyl Violet acetate |
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| GT8071-1G | Glentham Life Sciences | 1 g | EUR 78 |
Cresyl Violet acetate |
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| GT8071-5G | Glentham Life Sciences | 5 g | EUR 174 |
 Gentian Violet Solution |
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| GVS125 | ScyTek Laboratories | 125 ml | EUR 84 |
Gentian Violet Solution |
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| GVS500 | ScyTek Laboratories | 500 ml | EUR 130 |
Gentian Violet Solution |
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| GVS999 | ScyTek Laboratories | 1000 ml | EUR 178 |
 250UL MAXYMUM RECOVERY VIOLET TIPS FOR V-PREP. |
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| VT-250-L-R | CORNING | 960/pk | EUR 247 |
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Description: Robotic Tips; Maxymum Recovery Robotic Tips - Axygen |
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 250UL PRE-STERILIZED VIOLET TIPS FOR V-PREP |
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| VT-250-R-S | CORNING | 960/pk | EUR 275 |
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Description: Robotic Tips; Robotic tips - Axygen |
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 C.I.Basic Violet 11:1 |
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| abx180051-25g | Abbexa | 25 g | EUR 342 |
) Ethyl Violet (C.I. 42600) |
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| GT2065-100G | Glentham Life Sciences | 100 g | EUR 98 |
Ethyl Violet (C.I. 42600) |
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| GT2065-25G | Glentham Life Sciences | 25 g | EUR 54 |
 Nuclear Violet™ LCS1 |
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| 17543 | AAT Bioquest | 0.5 ml | EUR 132 |
 ETHYL VIOLET AZIDE BROTH |
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| E05-105-10kg | Alphabiosciences | 10 kg | EUR 1302 |
ETHYL VIOLET AZIDE BROTH |
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| E05-105-2kg | Alphabiosciences | 2kg | EUR 322 |
ETHYL VIOLET AZIDE BROTH |
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| E05-105-500g | Alphabiosciences | 500 g | EUR 124 |
 VIOLET RED BILE AGAR |
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| V22-100-10kg | Alphabiosciences | 10 kg | EUR 1013 |
VIOLET RED BILE AGAR |
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| V22-100-2Kg | Alphabiosciences | 2 Kg | EUR 259 |
VIOLET RED BILE AGAR |
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| V22-100-500g | Alphabiosciences | 500 g | EUR 107 |
 165UL VIOLET PRE-STERILIZED FILTERED TIPS FOR V-PREP |
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| VTF-165-R-S | CORNING | 960/pk | EUR 360 |
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Description: Robotic Tips; Filter Tips Robotic Tips - Axygen |
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 20UL VIOLET PRE-STERILIZED FILTERED TIPS FOR V-PREP |
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| VTF-20-R-S | CORNING | 960/pk | EUR 360 |
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Description: Robotic Tips; Filter Tips Robotic Tips - Axygen |
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) ELISA kit for Human CLC (Charcot Leyden Crystal Protein) |
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| ELK3140 | ELK Biotech | 1 plate of 96 wells | EUR 432 |
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Description: A sandwich ELISA kit for detection of Charcot Leyden Crystal Protein from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
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ELISA kit for Human CLC (Charcot Leyden Crystal Protein) |
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| E-EL-H1517 | Elabscience Biotech | 1 plate of 96 wells | EUR 534 |
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Description: A sandwich ELISA kit for quantitative measurement of Human CLC (Charcot Leyden Crystal Protein) in samples from Serum, Plasma, Cell supernatant |
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) CLIA kit for Human CLC (Charcot Leyden Crystal Protein) |
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| E-CL-H0976 | Elabscience Biotech | 1 plate of 96 wells | EUR 584 |
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Description: A sandwich CLIA kit for quantitative measurement of Human CLC (Charcot Leyden Crystal Protein) in samples from Serum, Plasma, Cell supernatant |
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) ELISA kit for Mouse CLC (Charcot Leyden Crystal Protein) |
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| ELK6116 | ELK Biotech | 1 plate of 96 wells | EUR 432 |
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Description: A sandwich ELISA kit for detection of Charcot Leyden Crystal Protein from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
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 Charcot-Leyden Crystal Protein |
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| 20-abx261451 | Abbexa |
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 1.5ML BOIL-PROOF MICROTUBES. VIOLET |
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| MCT-150-V | CORNING | 500/pk | EUR 110 |
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Description: Micro Tubes; Micro Tubes Push Cap- Axygen |
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 2.0ML BOIL-PROOF MICROTUBES. VIOLET |
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| MCT-200-V | CORNING | 500/pk | EUR 116 |
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Description: Micro Tubes; Micro Tubes Push Cap- Axygen |
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) Cresyl Echt Violet Solution (0.1%) |
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| CEA125 | ScyTek Laboratories | 125 ml | EUR 71 |
Cresyl Echt Violet Solution (0.1%) |
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| CEA500 | ScyTek Laboratories | 500 ml | EUR 96 |
Cresyl Echt Violet Solution (0.1%) |
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| CEA999 | ScyTek Laboratories | 1000 ml | EUR 127 |
) Cresyl Echt Violet (1.0%, Aqueous) |
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| CEB500 | ScyTek Laboratories | 500 ml | EUR 96 |
Cresyl Echt Violet (1.0%, Aqueous) |
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| CEB999 | ScyTek Laboratories | 1000 ml | EUR 124 |
) Methyl Violet 2B (C.I. 42535) |
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| GT4491-100G | Glentham Life Sciences | 100 g | EUR 229 |
Methyl Violet 2B (C.I. 42535) |
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| GT4491-25G | Glentham Life Sciences | 25 g | EUR 86 |
) Acid Violet 17 (C.I. 42650) |
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| GT4801-50G | Glentham Life Sciences | 50 g | EUR 81 |
 mFluor™ Violet 450 acid |
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| 1140 | AAT Bioquest | 5 mg | EUR 219 |
 mFluor™ Violet 510 acid |
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| 1141 | AAT Bioquest | 5 mg | EUR 306 |
 mFluor™ Violet 540 acid |
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| 1142 | AAT Bioquest | 5 mg | EUR 306 |
 mFluor™ Violet 500 SE |
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| 1149 | AAT Bioquest | 1 mg | EUR 219 |
 mFluor™ Violet 450 SE |
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| 1150 | AAT Bioquest | 1 mg | EUR 219 |
 mFluor™ Violet 510 SE |
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| 1151 | AAT Bioquest | 1 mg | EUR 219 |
 mFluor™ Violet 540 SE |
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| 1152 | AAT Bioquest | 1 mg | EUR 219 |
 250UL PRE-STERILIZED VIOLET TIPS FOR V-PREP. WITH BOTTOM LABEL |
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| VT-250-R-LAB-S | CORNING | 960/pk | EUR 275 |
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Description: Robotic Tips; Robotic tips - Axygen |
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 165UL VIOLET MAXYMUM RECOVERY PRE-STERILIZED FILTERED TIPS FOR V-PREP |
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| VTF-165-L-R-S | CORNING | 960/pk | EUR 418 |
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Description: Robotic Tips; Max Rec Robotic Filter Tips - Axygen |
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 mFluor™ Violet 450-streptavidin conjugate |
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| 16930 | AAT Bioquest | 100 ug | EUR 132 |
 mFluor™ Violet 510-streptavidin conjugate |
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| 16931 | AAT Bioquest | 100 ug | EUR 132 |
 mFluor™ Violet 540-streptavidin conjugate |
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| 16932 | AAT Bioquest | 100 ug | EUR 132 |
 VIOLET RED BILE GLUCOSE LACTOSE AGAR |
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| V22-105-10kg | Alphabiosciences | 10 kg | EUR 1099 |
VIOLET RED BILE GLUCOSE LACTOSE AGAR |
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| V22-105-2Kg | Alphabiosciences | 2 Kg | EUR 278 |
VIOLET RED BILE GLUCOSE LACTOSE AGAR |
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| V22-105-500g | Alphabiosciences | 500 g | EUR 112 |
 Pesticidal crystal protein cry1Ac Antibody |
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| 20-abx110511 | Abbexa |
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 Pesticidal crystal protein cry1Ab Antibody |
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| 20-abx110512 | Abbexa |
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Pesticidal crystal protein cry1Ac Antibody |
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| 20-abx110513 | Abbexa |
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 Pesticidal crystal protein cry3Aa Antibody |
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| 20-abx110515 | Abbexa |
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Pesticidal crystal protein cry1Ab Antibody |
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| 20-abx110516 | Abbexa |
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) Crystal Ponceau 6R (C.I. 16250) |
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| GT6539-100G | Glentham Life Sciences | 100 g | EUR 269 |
Crystal Ponceau 6R (C.I. 16250) |
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| GT6539-25G | Glentham Life Sciences | 25 g | EUR 134 |
Crystal Ponceau 6R (C.I. 16250) |
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| GT6539-5G | Glentham Life Sciences | 5 g | EUR 66 |
) Pesticidal crystal protein cry1Fb (cry1Fb) |
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| 1-CSB-YP528987BRS | Cusabio |
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Description: Recombinant Pesticidal crystal protein cry1Fb(cry1Fb),partial expressed in Yeast |
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, Red Fluorescence) EZClick? Palmitoylated Protein Assay Kit (FACS/Microscopy), Red Fluorescence |
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| K416-100 | Biovision | EUR 490 | |
, Red Fluorescence) EZClick? Stearoylated Protein Assay Kit (FACS/Microscopy), Red Fluorescence |
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| K418-100 | Biovision | EUR 490 | |
The outcomes present promising adsorption properties amongst at present obtainable adsorbents (the entire equilibrium adsorption capability was 24.5 mg/g) from the sludge with passable nanoadsorbent reusability and its speedy removing. The soundness of the nanoadsorbent within the sludge, an vital however typically uncared for sensible parameter for environment friendly removing, was verified. This work opens up new prospects for the event of high-quality magnetic nanoadsorbents for steel pollution utilized in varied sophisticated environmental fields and permits waste restoration.