Lichenized fungi normally develop advanced, stratified morphologies by an intricately balanced dwelling along with their algal companions, however a number of species are recognized to type solely kind of free associations with algae. These borderline lichens are nonetheless little explored though they may inform us about early levels of lichen evolution. We studied the affiliation of the extraordinarily halotolerant fungus Hortaea werneckii with the alga Dunaliella atacamensis, found in a cave within the Atacama Desert (Chile), and with D. salina, frequent inhabitant of saltern brines. D. atacamensis kinds small colonies, during which cells of H. werneckii might be regularly noticed, whereas such interplay has not been noticed with D. salina.
As symbiotic interactions between Dunaliella and Hortaea haven’t been reported, we carried out a sequence of co-cultivation experiments to examine whether or not these species may work together and develop extra distinct lichen-like symbiotic constructions. We arrange co-cultures between axenic strains of Hortaea werneckii (remoted each from Mediterranean salterns and from the Atacama cave) and isolates of D. atacamensis (from the Atacama cave) and D. salina (remoted from Mediterranean salterns). Though we used totally different progress media and cultivation approaches, vibrant discipline and SEM microscopy analyses didn’t point out any mutual results in these experiments. We talk about the implications for fungal algal interactions alongside the transition from algal exploiters to lichen symbioses.
Tea leaves possess quite a few unstable natural compounds (VOC) that contribute to tea’s attribute aroma. Some elements of tea VOC had been recognized to exhibit antimicrobial exercise; nonetheless, their impression on micro organism stays elusive. Right here, we confirmed that the VOC of contemporary aqueous tea leaf extract, recovered by hydrodistillation, promoted cell division and tryptophan-dependent indole-3-acetic acid (IAA) manufacturing in Pseudomonas sp. NEEL19, a solvent-tolerant isolate of the tea phylloplane. 1-octanol was recognized as one of many accountable volatiles stimulating cell division, metabolic change, swimming motility, putative pili/nanowire formation and IAA manufacturing, by gasoline chromatography-mass spectrometry, microscopy and partition petri dish tradition analyses.
The bacterial metabolic responses together with IAA manufacturing elevated below 1-octanol vapor in a dose-dependent method, whereas direct-contact in liquid tradition didn’t elicit such response. Thus, unstable 1-octanol emitting from tea leaves is a possible modulator of cell division, colonization and phytohormone manufacturing in NEEL19, probably influencing the tea aroma.
Rearrangement of Actin Cytoskeleton by Zika Virus An infection Facilitates Blood-Testis Barrier Hyperpermeability
In recent times, varied critical illnesses brought on by Zika virus (ZIKV) have made it unimaginable to be ignored. Confirmed existence of ZIKV in semen and sexually transmission of ZIKV steered that it might probably break the blood-testis barrier (BTB), or Sertoli cell barrier (SCB). Nonetheless, little is thought in regards to the underlying mechanism. On this research, interplay between actin, an essential part of the SCB, and ZIKV envelope (E) protein area III (EDIII) was inferred from co-immunoprecipitation (Co-IP) liquid chromatography-tandem mass spectrometry (LC-MS/MS) evaluation.
Confocal microscopy confirmed the function of actin filaments (F-actin) in ZIKV an infection, throughout which a part of the stress fibers, the bundles that constituted by paralleled actin filaments, had been disrupted and offered within the cell periphery. Colocalization of E and reorganized actin filaments within the cell periphery of transfected Sertoli cells suggests a participation of ZIKV E protein in ZIKV-induced F-actin rearrangement. Perturbation of F-actin by cytochalasin D (CytoD) or Jasplakinolide (Jas) enhanced the an infection of ZIKV. Extra importantly, the transepithelial electrical resistance (TEER) of an in vitro mouse SCB (mSCB) mannequin declined with the development of ZIKV an infection or overexpression of E protein.
Co-IP and confocal microscopy analyses revealed that the interplay between F-actin and tight junction protein ZO-1 was decreased after ZIKV an infection or E protein overexpression, highlighting the function of E protein in ZIKV-induced disruption of the BTB. We conclude that the interplay between ZIKV E and F-actin results in the reorganization of F-actin community, thereby compromising BTB integrity.
Matrix stiffness promotes glioma cell stemness by activating BCL9L/Wnt/β-catenin signaling
 solution for western blot) Single solution TMB substrate (membrane) solution for western blot |
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| 80110 | Alpha Diagnostics | 100 mls | EUR 225 |
 Stop solution for TMB substrate |
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| 10-9500-50 | Medicago | 50 ml | EUR 54 |
) Differentiating Solution (For Orcein Stain) |
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| DSA125 | ScyTek Laboratories | 125 ml | EUR 71 |
Differentiating Solution (For Orcein Stain) |
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| DSA500 | ScyTek Laboratories | 500 ml | EUR 122 |
 BCIP/NBT Solution for IHC |
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| B3007-005 | GenDepot | 50ml | EUR 134 |
BCIP/NBT Solution for IHC |
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| B3007-010 | GenDepot | 100ml | EUR 188 |
BCIP/NBT Solution for IHC |
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| B3007-050 | GenDepot | 500ml | EUR 475 |
) Oxidizer Solution (For Steiner's Silver Stain) |
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| SOS125 | ScyTek Laboratories | 125 ml | EUR 76 |
Oxidizer Solution (For Steiner's Silver Stain) |
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| SOS500 | ScyTek Laboratories | 500 ml | EUR 111 |
 Sodium Pyruvate Solution, 100 mM Solution |
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| CCM1753-100 | Bio Basic | 100 mL | EUR 60.84 |
|
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 SDS Solution |
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| 2102-100 | Biovision | EUR 137 | |
 EDTA Solution |
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| 2103-100 | Biovision | EUR 137 | |
 TRIMMING SOLUTION |
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| 99-676-CM | CORNING | 1 L /pk | EUR 88 |
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Description: Specialty Media; Islet products |
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 AlphaBioCoat Solution |
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| AC001 | Neuromics | 20 ml | EUR 271 |
 Borax Solution |
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| BOR015 | ScyTek Laboratories | 15 ml | EUR 56 |
Borax Solution |
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| BOR125 | ScyTek Laboratories | 125 ml | EUR 79 |
Borax Solution |
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| BOR500 | ScyTek Laboratories | 500 ml | EUR 119 |
Borax Solution |
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| BOR999 | ScyTek Laboratories | 1000 ml | EUR 183 |
 Stop Solution |
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| F006 | Cygnus Technologies | 12 ml | EUR 180 |
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Description: Stop Solution by Cygnus Technologies is available in Europe via Gentaur. |
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 Prehybridization Solution |
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| K2191050-1 | Biochain | 5 ml | EUR 137 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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 Hybridization Solution |
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| K2191050-2 | Biochain | 5 ml | EUR 157 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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 Blocking Solution |
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| K2191050-8 | Biochain | 250 ul | EUR 137 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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 Orcein Solution |
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| OAA125 | ScyTek Laboratories | 125 ml | EUR 66 |
Orcein Solution |
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| OAA500 | ScyTek Laboratories | 500 ml | EUR 109 |
Orcein Solution |
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| OAA999 | ScyTek Laboratories | 1000 ml | EUR 148 |
 Methenamine Solution |
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| MET125 | ScyTek Laboratories | 125 ml | EUR 64 |
Methenamine Solution |
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| MET500 | ScyTek Laboratories | 500 ml | EUR 95 |
Methenamine Solution |
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| MET999 | ScyTek Laboratories | 1000 ml | EUR 132 |
 DTT Solution |
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| R-1104 | EpiGentek | 1 ml, 500 mM | EUR 109.45 |
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Description: fast delivery possible |
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 Tartrazine Solution |
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| TZQ125 | ScyTek Laboratories | 125 ml | EUR 64 |
Tartrazine Solution |
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| TZQ500 | ScyTek Laboratories | 500 ml | EUR 98 |
Tartrazine Solution |
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| TZQ999 | ScyTek Laboratories | 1000 ml | EUR 127 |
 Schiff's Solution |
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| SRF030 | ScyTek Laboratories | 30 ml | EUR 64 |
Schiff's Solution |
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| SRF250 | ScyTek Laboratories | 250 ml | EUR 77 |
Schiff's Solution |
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| SRF3800 | ScyTek Laboratories | 1 Gal. | EUR 345 |
Schiff's Solution |
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| SRF500 | ScyTek Laboratories | 500 ml | EUR 103 |
Schiff's Solution |
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| SRF999 | ScyTek Laboratories | 1000 ml | EUR 149 |
 Denaturing solution |
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| DE540 | Bio Basic | 1L | EUR 61.31 |
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 PMA Enhancer for Gram Negative Bacteria, 5X Solution |
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| 31038 | Biotium | 16ml | EUR 132 |
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Description: Minimum order quantity: 1 unit of 16ml |
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 concentrate (10X)) Stop solution for TMB substrate (ELISA) concentrate (10X) |
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| 80100 | Alpha Diagnostics | 50 ml | EUR 164 |
) HANKS BALANCED SALT SOLUTION 1X, (MODIFIED FOR ISLETS) |
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| 99-597-CM | CORNING | 1 L /pk | EUR 57 |
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Description: Specialty Media; Islet products |
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) Tissue RNA Storage Solution (for future RNA extraction) |
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| W0592-100 | 101Bio | NULL | EUR 0 |
Tissue RNA Storage Solution (for future RNA extraction) |
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| W0592-20 | 101Bio | NULL | EUR 0 |
Tissue RNA Storage Solution (for future RNA extraction) |
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| W0592-500 | 101Bio | NULL | EUR 0 |
, Green Fluorescence) EZClick? Myristoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence |
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| K497-100 | Biovision | EUR 490 | |
, Green Fluorescence) EZClick? Palmitoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence |
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| K452-100 | Biovision | EUR 490 | |
, Green Fluorescence) EZClick? Stearoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence |
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| K453-100 | Biovision | EUR 490 | |
, Red Fluorescence) EZClick? Palmitoylated Protein Assay Kit (FACS/Microscopy), Red Fluorescence |
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| K416-100 | Biovision | EUR 490 | |
, Red Fluorescence) EZClick? Stearoylated Protein Assay Kit (FACS/Microscopy), Red Fluorescence |
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| K418-100 | Biovision | EUR 490 | |
 Gentamicin Solution, Gentamicin Solution, 50 mg/mL |
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| CCM1123-010 | Bio Basic | 10 mL | EUR 64.05 |
|
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 Holder for Plasmid Midi, Maxi and Maxi plus, Ion Exchange column |
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| FAPDE-holder-for-ion-exchange | Favorgen | 1 prep | EUR 158 |
 Concentrating Solution for Sirius Red Total Collagen Detection Kit |
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| 90626 | Chondrex | 50 ml | EUR 67.4 |
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Description: Concentrating Solution for Sirius Red Total Collagen Detection Kit |
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 Cumate Solution, high concentration, 10,000x for use with PiggyBac |
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| PBQM100A-1 | SBI | 500 ul | EUR 158 |
|
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, Red Fluorescence) EZClick? Global Protein Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence |
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| K715-100 | Biovision | EUR 550 | |
, Red Fluorescence) EZClick? Global Phospholipid Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence |
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| K717-100 | Biovision | EUR 512 | |
, Red Fluorescence) EZClick? Global RNA Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence |
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| K718-100 | Biovision | EUR 533 | |
, Green Fluorescence) EZClick? Global Protein Synthesis Assay Kit (FACS/Microscopy), Green Fluorescence |
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| K459-100 | Biovision | EUR 550 | |
EZClick? Global RNA Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence |
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| K462-100 | Biovision | EUR 533 | |
 HYGROMYCIN B SOLUTION |
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| 30-240-CR | CORNING | 20 mL/pk | EUR 157 |
|
Description: Media Catalog; Cell Culture Reagents |
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 Ponceau S Solution |
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| 22001 | Biotium | 1L | EUR 125 |
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Description: Minimum order quantity: 1 unit of 1L |
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) HEPES SOLUTION (1M) |
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| 25-060-CI | CORNING | 100 mL/pk | EUR 104 |
|
Description: Media Catalog; Cell Culture Reagents |
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 Acrylamide Solution 30% |
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| A067-1L | TOKU-E | 1 L | EUR 103 |
Acrylamide Solution 30% |
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| A067-500Ml | TOKU-E | 500 ml | EUR 71 |
 Ringer Solution Tablets |
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| 09-1025-100 | Medicago | 100 tablets | EUR 70 |
 PROTEINASE K SOLUTION |
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| 501-PK | Viagen | 5 ml | EUR 152 |
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Description: Genomic PCR Grade Proteinase K Solution |
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 CometAssay Lysis Solution |
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| 4250-010-01 | Biotechne | 100 mL | EUR 157.8 |
 Alcian Yellow Solution |
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| ANY125 | ScyTek Laboratories | 125 ml | EUR 83 |
Alcian Yellow Solution |
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| ANY3800 | ScyTek Laboratories | 1 Gal. | EUR 454 |
Alcian Yellow Solution |
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| ANY500 | ScyTek Laboratories | 500 ml | EUR 123 |
Alcian Yellow Solution |
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| ANY999 | ScyTek Laboratories | 1000 ml | EUR 178 |
 solution) Tyloxapol (10% ) solution |
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| B1159-250 | Biovision | EUR 512 | |
Tyloxapol (10% ) solution |
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| B1159-50 | Biovision | EUR 175 | |
 Hematoxylin Counterstain Solution |
|||
| AR0005 | BosterBio | 12mL(for 120-180 assays) | EUR 65 |
 Ponceau S solution |
|||
| AR0142 | BosterBio | 12mL(10X) | EUR 60 |
 Astra Blue Solution |
|||
| AST125 | ScyTek Laboratories | 125 ml | EUR 104 |
Astra Blue Solution |
|||
| AST500 | ScyTek Laboratories | 500 ml | EUR 183 |
 DAB Differentiating Solution |
|||
| DDS500 | ScyTek Laboratories | 500 ml | EUR 109 |
 Crystal Violet Solution |
|||
| CVA500 | ScyTek Laboratories | 500 ml | EUR 106 |
Crystal Violet Solution |
|||
| CVA999 | ScyTek Laboratories | 1000 ml | EUR 143 |
 Stop/Elution Solution |
|||
| 85R-123 | Fitzgerald | 250 ml | EUR 155 |
|
Description: Elution or stop reagent for use in immunoassays |
|||
 Acrylamide 40% Solution |
|||
| A0416-050 | GenDepot | 495ml | EUR 165 |
 STOCK POLYSUCROSE SOLUTION |
|||
| 99-662-CVS | CORNING | 300 mL/pk | EUR 188 |
|
Description: Specialty Media; Islet products |
|||
 GRADIENT STOCK SOLUTION |
|||
| 99-674-CM | CORNING | 1 L /pk | EUR 614 |
|
Description: Specialty Media; Islet products |
|||
 PHASE I SOLUTION |
|||
| 99-678-CM | CORNING | 1 L /pk | EUR 105 |
|
Description: Specialty Media; Islet products |
|||
 PENTASTARCH, 10& SOLUTION |
|||
| 99-723-CM | CORNING | 1 L /pk | EUR 386 |
|
Description: Specialty Media; Islet products |
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 Stannous Tetrafluoroborate Solution |
|||
| 20-abx188890 | Abbexa |
|
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|
|||
 Rhodium nitrate solution |
|||
| 20-abx186014 | Abbexa |
|
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|
|||
 Propidium Iodide Solution |
|||
| abx090616-100tests | Abbexa | 100 tests | EUR 217 |
|
|||
 Phenol Red Solution |
|||
| CA013-010 | GenDepot | 100ml | EUR 69 |
 Sodium Pyruvate Solution |
|||
| CA017-010 | GenDepot | 100ml | EUR 72 |
 0.5M CaCl2 Solution |
|||
| CA059-100 | GenDepot | 100ml | EUR 80 |
 Carbol Fuchsin Solution |
|||
| CFZ125 | ScyTek Laboratories | 125 ml | EUR 79 |
Carbol Fuchsin Solution |
|||
| CFZ500 | ScyTek Laboratories | 500 ml | EUR 124 |
Carbol Fuchsin Solution |
|||
| CFZ999 | ScyTek Laboratories | 1000 ml | EUR 173 |
 Chromic Acid Solution |
|||
| CHR125 | ScyTek Laboratories | 125 ml | EUR 84 |
Chromic Acid Solution |
|||
| CHR500 | ScyTek Laboratories | 500 ml | EUR 143 |
Chromic Acid Solution |
|||
| CHR999 | ScyTek Laboratories | 1000 ml | EUR 226 |
 Cell Detachment Solution |
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| ADF001 | Neuromics | 50 ml | EUR 114 |
 Universal Coating Solution |
|||
| AC002 | Neuromics | 20 ml | EUR 297 |
 BCIP/INT Solution |
|||
| ACL050 | ScyTek Laboratories | 50 ml | EUR 79 |
BCIP/INT Solution |
|||
| ACL125 | ScyTek Laboratories | 125 ml | EUR 98 |
BCIP/INT Solution |
|||
| ACL500 | ScyTek Laboratories | 500 ml | EUR 205 |
BCIP/INT Solution |
|||
| ACL999 | ScyTek Laboratories | 1000 ml | EUR 319 |
 DAB Enhancer Solution |
|||
| ACM030 | ScyTek Laboratories | 30 ml | EUR 71 |
 BCIP/NBT Solution |
|||
| ACN050 | ScyTek Laboratories | 50 ml | EUR 79 |
BCIP/NBT Solution |
|||
| ACN125 | ScyTek Laboratories | 125 ml | EUR 117 |
BCIP/NBT Solution |
|||
| ACN500 | ScyTek Laboratories | 500 ml | EUR 260 |
BCIP/NBT Solution |
|||
| ACN999 | ScyTek Laboratories | 1000 ml | EUR 394 |
 Universal Detachment Solution |
|||
| AD002 | Neuromics | 100 ml | EUR 148 |
 MgCl2 Solution, 50mM |
|||
| BIO-37026 | Bioline | 3 x 1.2ml | Ask for price |
 Proteinase K Solution |
|||
| BIO-37084 | Bioline | 5ml @ 20mg/ml | Ask for price |
Proteinase K Solution |
|||
| BIO-37085 | Bioline | 5 x 5ml @ 20mg/ml | Ask for price |
 Congo Red Solution |
|||
| CRA030 | ScyTek Laboratories | 30 ml | EUR 74 |
Congo Red Solution |
|||
| CRA500 | ScyTek Laboratories | 500 ml | EUR 106 |
Congo Red Solution |
|||
| CRA999 | ScyTek Laboratories | 1000 ml | EUR 143 |
 Aniline Blue Solution |
|||
| ABP125 | ScyTek Laboratories | 125 ml | EUR 72 |
Aniline Blue Solution |
|||
| ABP500 | ScyTek Laboratories | 500 ml | EUR 106 |
Aniline Blue Solution |
|||
| ABP999 | ScyTek Laboratories | 1000 ml | EUR 133 |
) Hematoxylin Solution (5%) |
|||
| HSV250 | ScyTek Laboratories | 250 ml | EUR 133 |
Hematoxylin Solution (5%) |
|||
| HSV500 | ScyTek Laboratories | 500 ml | EUR 183 |
Hematoxylin Solution (5%) |
|||
| HSV999 | ScyTek Laboratories | 1000 ml | EUR 277 |
 Gentian Violet Solution |
|||
| GVS125 | ScyTek Laboratories | 125 ml | EUR 84 |
Gentian Violet Solution |
|||
| GVS500 | ScyTek Laboratories | 500 ml | EUR 130 |
Gentian Violet Solution |
|||
| GVS999 | ScyTek Laboratories | 1000 ml | EUR 178 |
 Proteinase K solution |
|||
| GZ5904-100ML | Glentham Life Sciences | 100 ml | EUR 907 |
Proteinase K solution |
|||
| GZ5904-25ML | Glentham Life Sciences | 25 ml | EUR 349 |
 RNA Stabilization Solution |
|||
| FARSS-001 | Favorgen | 100ml | EUR 208 |
 Eosin-Phloxine Solution |
|||
| EPN-10000 | ScyTek Laboratories | 10 L | EUR 383 |
Eosin-Phloxine Solution |
|||
| EPN3800 | ScyTek Laboratories | 1 Gal. | EUR 210 |
Eosin-Phloxine Solution |
|||
| EPN500 | ScyTek Laboratories | 500 ml | EUR 71 |
Eosin-Phloxine Solution |
|||
| EPN999 | ScyTek Laboratories | 1000 ml | EUR 87 |
 Exosome precipitation Solution |
|||
| EPStep | ImmunoStep | 12 ml | EUR 277.5 |
) Formalin Solution (20%) |
|||
| FRL125 | ScyTek Laboratories | 125 ml | EUR 63 |
Formalin Solution (20%) |
|||
| FRL500 | ScyTek Laboratories | 500 ml | EUR 82 |
Formalin Solution (20%) |
|||
| FRL999 | ScyTek Laboratories | 1000 ml | EUR 101 |
 RNase A Solution |
|||
| G118 | ABM | 1.0 ml; 10 mg/ml | EUR 102 |
 Sodium silicate solution |
|||
| GK8776-100ML | Glentham Life Sciences | 100 ml | EUR 40 |
Sodium silicate solution |
|||
| GK8776-1L | Glentham Life Sciences | 1 l | EUR 66 |
Sodium silicate solution |
|||
| GK8776-250ML | Glentham Life Sciences | 250 ml | EUR 46 |
Sodium silicate solution |
|||
| GK8776-500ML | Glentham Life Sciences | 500 ml | EUR 52 |
Sodium silicate solution |
|||
| GK8776-5L | Glentham Life Sciences | 5 l | EUR 120 |
 Gram's Decolorizer Solution |
|||
| GDS125 | ScyTek Laboratories | 125 ml | EUR 72 |
Gram's Decolorizer Solution |
|||
| GDS500 | ScyTek Laboratories | 500 ml | EUR 101 |